T1346I fully rescues DNA damage sensitivity and RAD51 foci formation upon irradiation. Missense variant residues are boxed in red. Sequence alignment of BRC2, BRC4, and BRC7 repeats. RAD51 D187 residue is displayed in green. BRC4 T1526A is a previously characterized missense variant that disrupts RAD51 binding. Bottom Insets: predicted disruptions resulting from variants BRC2 (S1221P), BRC4 (T1526A), and BRC7 (T1980I). Top Inset: BRC2 (S1221), BRC4 (T1526), and BRC7 (T1980) overlayed to show conservation. Homology model comparison of BRC2 (orange) and BRC7 (yellow) based on the BRC4 (pink) structure. ( C) Structural models based on 1N0W structure. FxTASGK is a consensus motif important for RAD51 interactions depicted below BRC2 and BRC7. Uniprot and ClustalX (70% threshold for shading) were used for the alignment. Residue corresponding to the missense variant is indicated in pink and conserved residues flanking the missense residue are indicated in light grey. ( B) Multiple sequence alignment of BRCA2 amino acids flanking each missense variant from different organisms: S1221P (BRC2), T1346I (Spacer BRC2-3), and T1980I (BRC7). BRCA2 missense variants used in this study are indicated. ( A) BRCA2 protein schematic depicting domain organization: 2XMBP tag, N-terminus, BRC repeats, DNA binding domain (DBD), and C-terminal domain (CTD). You are solely responsible for adequate protection and backup of the data and equipment used in connection with using software ImageQuant TL.S1221P and T1980I are structurally predicted to disrupt BRC folding and RAD51 binding. The use of the software and any damage done to your systems. PCWin has not developed this software ImageQuant TL and in no way responsible for PCWin free download center makes no representations as to the content of ImageQuant TL version/build 1.1 is accurate, complete, virus free or do not infringe the rights There are inherent dangers in the use of any software available for download on the Internet. ImageQuant TL 1.1 download version indexed from servers all over the world. It is structured around frequently used applications and is an essential. ImageQuant TL is the most powerful, flexible and competitive general image analysis software available to the biotech community. Use one consistent and intuitive interface.

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